Abstract：An optimized CTAB method for total RNA extraction was presented for use in accurately quantifying components of animal origin and fighting against adulterated food in the market as a sample preparation method. The use of the CTAB method to extract total RNA from pork was investigated with respect to sample amount, dissociation time, precipitation time and RNase treatment. As a result, a fast and efficient method for total RNA extraction was achieved by selecting sample amount below 0.05 g, 0.5 h dissociation, 0.5h precipitation and non-RNase treatment. The method showed a good linear relationship （R2 = 0.9972） and stability （SD = 0.18）.
2011, Vol. 25 
