Abstract：In the present study, a two dimensional （2-DE） gel electrophoresis system was established and optimized for pork liver proteome. The sample preparation was achieved using ultracentrifugation and cleanup kits and ready IPG strips ImmobilineTM DryStrip gel （ 17 cm and pH5 - 8） were used at a sample loading level of 120/a L, resulting in a matching rate of 76% and 1412 ± 8 protein spots. According to the total blindness method, accuracy and repeatability tests were performed on different pork liver samples, showing a matching rate of over 70% and spots having a relative mobility within the laboratory control standards. Therefore, protein spots having statistical significance and allowing quantitative analysis could be screened out and be used for the differentiation of pork liver samples. In conclusion, the established system can allow 2-DE gel electrophoresis of sample pork liver samples.
2011, Vol. 25 
